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Induksi apoptosis jalur mitokondria sel kanker Hela oleh secretome (Laporan Penelitian)


Oleh : Elisabeth Pricilia Sunata

Info Katalog

Nomor Panggil : 616.07 Sun i

Penerbit : FKG - Usakti

Kota Terbit : Jakarta

Tahun Terbit : 2014

Pembimbing 1 : drg. Ferry Sandra, Ph.D.

Subyek : Anatomic Pathologist

Kata Kunci : umbilical cord blood, mesenchymal stem cell, secretome, apoptosis, cancer cell


File Repositori
No. Nama File Ukuran (KB) Status
1. 2014_TA_KG_04010068_Daftar-Pustaka.pdf 1385.86
2. 2014_TA_KG_04010068_Bab-7.pdf 592.4
3. 2014_TA_KG_04010068_Bab-6.pdf 818.33
4. 2014_TA_KG_04010068_Bab-5.pdf 2268
5. 2014_TA_KG_04010068_Bab-4.pdf 1036.95
6. 2014_TA_KG_04010068_Bab-3.pdf 801.4
7. 2014_TA_KG_04010068_Bab-2.pdf 1920.49
8. 2014_TA_KG_04010068_Bab-1.pdf 704.22
9. 2014_TA_KG_04010068_Halaman-Judul.pdf 1405.27

S Sel puca masekim (SPM) merupakan sel puca dewasa yang bersifat multipoten dan dapat diperoleh dalam jumlah yang banyak dari darah tali pusat (DTP). Banyak literatur yang menyatakan bahwa SPM dapat menginduksi apoptosis sel kanker. Namun banyak juga yang menyatakan bahwa SPM dapat mendukung pertumbuhan kanker. Penelitian ini dilakukan untuk menyelidiki kemampuan anti-kanker SPM-DTP terhadap lini sel kanker servikal (Hela).Pda penelitian ini sel Hela diberikan perlakuan secretome yang terkandung dalam conditioned medium (CM) yang diperoleh dari kultur SPM-DTP pada konsentrasi 0,2%,2%, 20% selama 24 atau 48 jam. Sel Hela yang mati akibat perlakuan tersebut dihitung dalam analis viabilitas sel dengan pewarnaan trypan blue.Transisi potensial tranmembran mitokondria (PTM) yang terjadi selama apoptosis dideteksi dalam analisis AѱM dengan pewarnaan DiOC₆ menggunakan flow cytometer. Hasil analisis viabilitas sel menunjukan bahwa CM SPM-DTP menginduksi kematian sel Hela, dan dijumpai peningkatan sel Hela yang yang mati seiring dengan peningkatan konsentrasi dan waktu perlakuan.

M Mesenchymal stem cells (MSCs) are multipotent adult stem cells that can be isolated from umbilical cord blood (UCB). Until now, the anti-cancer effect of MSCs still remains controversial between scientists. Some evidences demonstrate that MSCs can induce apoptosis of cancer cells. However, there are also studies that show MSCs may favor tumor growth. Based on this unclear effect of MSCs on tumors, this study is therefore initiated to investigate the anti-cancer effect of secretome contained in UCB-MSCs conditioned medium (CM) on cervical cancer cell line HeLa in vitro. Hela cells were treated with UCB-MSCs CM in various concentrations (0.2%, 2%, and 20%) for 24 or 48 hours. The count of dead HeLa cells was evaluated by cell viability analysis using trypan blue. The transition of mitochondrial transmembrane potential (TMP) which occurs during apoptosis was assayed by ΔΨM analysis using DiOC6 with flow cytometer. Cell viability analysis showed that death of HeLa cell was induced by UCB-MSCs CM, and an increase in concentrations and treatment time concomitantly resulted in increased number of dead HeLa cells. Treatment of HeLa cells with UCB-MSCs CM 0.2% for 24 hours was sufficient to induce death of HeLa cells. ΔΨM analysis showed the decrease of TMP, indicating an increase in mitochondrial membrane permeability of HeLa cells These results demonstrated that secretome contained in UCB-MSCs CM had the ability to induce HeLa cell apoptosis via mitochondrial pathway.

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