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Perbandingan Pengaruh Ekstrak Daun Sirsak (annona Muricata L.) Fraksi Etanol Dan Etil Asetat Terhadap Zona Hambat Tumbuh Streptococcus Mutans (laporan Penelitian)

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Oleh : Niajeng Nayenggita

Info Katalog

Nomor Panggil : 571 Nay p

Penerbit : FKG - Usakti

Kota Terbit : Jakarta

Tahun Terbit : 2015

Pembimbing 1 : drg. Allfred Pakpahan, M.Si.

Pembimbing 2 : -

Subyek : Biology

Kata Kunci : Soursop leaf extract, ethanol, ethyl acetate,diameter of inhibition zone, streptococcus mutans


File Repositori
No. Nama File Ukuran (KB) Status
1. 2015_TA_KG_04011165_Halaman-Judul.pdf 742.52

T Tanaman sirsak (Annona muricata L.) merupakan tanaman yang mudah dan banyak ditemukan di Indonesia. Selain buahnya yang lezat untuk dikonsumsi, daunnya sering dimanfaatkan sebagai bahan tanaman obat. Daun sirsak diteliti memiliki kandungan flavonoid, alkaloid dan senyawa annonaceous acetogenin yang berfungsi sebagai antibakteri. Salah satu bakteri penyebab karies gigi adalah Streptococcus mutans. Penelitian ini menggunakan ekstrak daun sirsak dengan pelarut etanol dan etil asetat dalam konsentrasi 5%, 10%, dan 20% yang diuji efek antibakterinya terhadap Streptococcus mutans. Klorheksidin dan tetrasiklin digunakan sebagai kontrol positif dan akuades sebagai kontrol negatif. Ekstrak diuji daya hambatnya dengan metode difusi pada media agar darah domba dan diinkubasi selama 24-48 jam. Pengukuran dan pencatatan diameter zona hambat dilakukan setiap 24 jam. Hasil yang diperoleh menunjukkan ekstrak etanol berdiameter 9,25 mm, 10,95 mm, 13,2 mm dan ekstrak etil asetat menghasilkan 10,5 mm, 12,71 mm, 15,63 mm pada 24 jam pertama, berurutan untuk konsentrasi 5%, 10% dan 20%. Pengukuran setelah 48 jam ekstrak etanol menjadi 10,37 mm, 11,75 mm, 14,2 mm dan etil asetat menjadi 11,17 mm, 13,33 mm, dan 16,5 mm. Ekstrak etil asetat 20% menghasilkan diameter paling besar dan tidak jauh berbeda dengan klorheksidin yang berdiameter 15,35 mm pada 24 jam 16,28 mm pada 48 jam. Seluruh diameter zona hambat diuji statistik dengan menggunakan one-way ANOVA dan menghasilkan nilai p<0,05 yang berarti terdapat perbedaan yang bermakna pada seluruh bahan uji. Dapat disimpulkan bahwa kedua ekstrak berpotensi menghambat pertumbuhan bakteri Streptococcus mutans dan ekstrak etil asetat menghasilkan zona hambat yang lebih besar.

S Soursop plant (Annona muricata L.) is a plant easily found in great quantities in Indonesia. Its fruit is delicious for consumption, while its leaves are often used for a medicinal purpose. Soursop leaves reportedly contains several important compounds such as flavonoid, alkaloid and annonaceous acetogenin which can function as antibacterial substances. One of the bacteria causing dental caries is Streptococcus mutans. This research used the soursop leaf extract diluted in the ethanol and the ethyl acetate in a concentration of 5%, 10%, and 20% to investigate its antibacterial effect against Streptococcus mutans. Chlorhexidine and tetracycline was used as a positive control and distilled water was used as a negative control. The extract was tested for its inhibitory effects using the diffusion method on a medium of sheep-blood agar under incubation for 24-48 hours. The diameters of the inhibition zone were measured and recorded every 24 hours. In the first 24 hours, the result showed that the ethanol extracts yielded respectively 9.25 mm, 10.95 mm, 13.2 mm diameter of the inhibition zone for the controlled concentration of 5%, 10% and 20%. Whereas the ethyl acetate extracts yielded respectively 10.5 mm, 12.71 mm, 15.63 mm diameter of the inhibition zone for the controlled concentration of 5%, 10% and 20%. Measurement after 48 hours showed that the ethanol extracts yielded 10.37 mm, 11.75 mm, 14.2 mm diameter respectively, whereas the ethyl acetate extracts yielded 11.17 mm, 13.33 mm, and 16.5 mm diameter respectively. The 20% ethyl acetate extract produced the greatest diameter as much as of the one produced by chlorhexidine which has 15,35 mm after 24 hours and 16,28 mm after 48 hours. One-way ANOVA statistical tool was used to test the data of the diameters of the inhibition zone. The statistical test showed values of p<0.05, which mean that the results of all tested materials were significantly different. It is concluded that both extracts potentially inhibit the growth of Streptococcus mutans and the ethyl acetate extracts produce greater inhibition zones.

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