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Perbandingan Ekstrak Kulit Pisang (musa Paradisiaca) Fraksi Etanol Dan Etil, Asetat Terhadap Zona Hambat Pertumbuhan Zona Hambat Pertumbuhan Staphylococcus Aureus (laporan Penelitian)


Oleh : Urai Gery Aryandi

Info Katalog

Nomor Panggil : 612.3 ARY p

Penerbit : FKG - Usakti

Kota Terbit : Jakarta

Tahun Terbit : 2016

Pembimbing 1 : Drs. Alfred Pakpahan, M.Si.

Subyek : Biology oral

Kata Kunci : Banana peel extract, ethanol, erthyl acectate, inhibiton zone, streptococcus mutans.


File Repositori
No. Nama File Ukuran (KB) Status
1. 2015_TA_KG_04012195_Halaman-Judul.pdf 1355.42

P Penelitian ini bertujuan untuk mengetahui perbandingan efek antibakteri ekstrak kulit M. paradisiaca terhadap pertumbuhan bakteri S. mutans dengan menggunakan konsentrasi 5%, 10%, dan 15% fraksi etanol dan etil asetat, klorheksidin 0,2%, tetrasiklin 2%, dan akuades digunakan sebagai kontrol. Ekstrak diuji daya hambatnya dengan metode difusi pada media blood plate agar dan diinkubasi selama 24-48 jam. Hasil pengukuran zona hambat ekstrak etanol 24 jam konsentrasi 5%, 10%, dan 15% adalah 27,78 mm, 32,73 mm, dan 33,22 mm. Sedangkan, fraksi etil asetat menghasilkan 30,85 mm, 33,38 mm, dan 33,29 mm. Pada pengukuran 48 jam ekstrak etanol menjadi 27,81 mm, 32,76 mm, 33,24 mm dan etil asetat menjadi 30,89 mm, 33,41 mm, 33,32 mm. Dari kedua fraksi menunjukkan diameter zona hambat terbaik pada fraksi etil asetat 10% yang dibuktikan melalui hasil uji statistik menggunakan one-way ANOVA (p<0.05), yang berarti terdapat pengaruh yang signifikan pada bahan ekstrak kulit M. paradisiaca terhadap bakteri S. mutans. Dapat disimpulkan bahwa kedua ekstrak berpotensi menghambat pertumbuhan bakteri S. mutans. Diharapkan penelitian tentang ekstrak kulit pisang dapat digunakan sebagai pengganti obat kumur berbahan kimia.

B Banana (Musa Paradisiaca) is plant that is easy and commonly found in Indonesia. Besides the fruit that delicious to be consumed, it also could be processed to other various ways such as fried and any other else. Despitefully, banana itself result a waste from its peel that is countless. Banana’s peel been studied has content of flavonoid, alkaloid, and tannin that is a compound of antibacterial. Streptococcus mutans is one of the bacteria that cause of dental caries and can be found in the mouth cavity. This aims of study is to understand the effect of antibacterial comparison extract the peel Musa paradisiaca on the growth of S. mutans bacteria using concentrated 5%, 10% and 15% fraction ethanol and ethyl acetate, chlorhexidine 0,2%, tetracycline 2% and aquadest as control. The extract have been tested its inhibition zone with diffusion method on a medium of blood agar and get incubation period for 24-48 hours. The results of inhibit zone in 24 hours of ethanol extracts concentrated 5%, 10%, 15% is 27,78 mm , 32,73 mm , and 33,22 mm. While fraction ethyl acetate shown 30,85 mm, 33,38 mm, and 33,29 mm. In 48 hours inhibition zone of the ethanol extract become 27,81 mm, 32,76 mm, 33,24 mm and ethyl acetate become 30,89 mm, 33,41 mm, 33,32 mm. From both fractions, are shown the best diameter of an inhibition zone of ethyl acetate 10%, which proved through statistical testing using one-way ANOVA (p<0.05), that means there is significant effect between ingredients in M. paradisiaca’s peel towards S. mutans bacteria. It is concluded both extract potential to inhibit the growth of S. mutans bacteria. It’s hoped that the research on banana peel extract can be used as a substitute for usage of a mouthwash.

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